formula:C 4 H 11 NO 3
package:25kg Paper Bag, 25kg Paper Drum, 25kg Cardboard Drum
Chinese name: Trishydroxymethylaminomethane
English name: Tris(hydroxymethyl)methyl aminomethane THAM
English alias: Tris base; 2-Amino-2-(hydroxymethyl)-1,3-propanediol; THAM; Trometamol
CAS No.: 77-86-1
Molecular formula: C4H11NO3
Linear formula: NH2C(CH2OH)3
Molecular weight: 121.14
MDL number: MFCD00004679
Beilstein number: 741883
EC number: 201-064-4
Insoluble matter: qualified
Loss on drying (110 ° C): ≤ 0.2%
Chloride (Cl): <0.005%
Sulfate (SO4): <0.005%
Iron (Fe): <0.0005%
First dissolve the Tris with a small amount of double distilled water (300~500ml), add HCl, adjust the pH to 7.6 with HCl (1N) or NaOH (1N), and finally add double distilled water to 1000ml. This liquid is a stock solution and stored in a 4 ° C refrigerator.
Note: The pH of Tris-Hcl varies with temperature, so it should be re-tested at room temperature. The results are reliable.
Tris buffers are not only widely used as solvents for nucleic acids and proteins, but also for many important uses. Tris is used for protein crystal growth under different pH conditions. The low ionic strength characteristics of Tris buffer can be used for the formation of intermediate fibers of C. elegans lamin. Tris is also one of the main components of protein electrophoresis buffers. In addition, Tris is also an intermediate for the preparation of surfactants, vulcanization accelerators and some drugs. Tris is also used as a titration standard.
Tris is a weak base with a pKa of 8.1 at room temperature (25 ° C); according to buffer theory, the effective buffer range of Tris buffer is between pH 7.0 and 9.2.
The pH of the aqueous solution of the Tris base is about 10.5, and hydrochloric acid is usually added to adjust the pH to a desired value to obtain a buffer of the pH. But also pay attention to the effect of temperature on the pKa of Tris.